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Quantitative Localization Microscopy: Effects of Photophysics and Labeling Stoichiometry
Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average nu...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4439177/ https://ncbi.nlm.nih.gov/pubmed/25992915 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0127989 |
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