Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons

Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to...

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Publicado en:mBio
Main Authors: Wetmore, Kelly M., Price, Morgan N., Waters, Robert J., Lamson, Jacob S., He, Jennifer, Hoover, Cindi A., Blow, Matthew J., Bristow, James, Butland, Gareth, Arkin, Adam P., Deutschbauer, Adam
Formato: Artigo
Idioma:Inglês
Publicado: American Society of Microbiology 2015
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4436071/
https://ncbi.nlm.nih.gov/pubmed/25968644
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.00306-15
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