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De novo protein structure determination from near-atomic resolution cryo-EM maps

We present a de novo model building approach that combines predicted backbone conformations with side-chain density-fit to accurately assign sequence into density maps. We show this method yields accurate models for six experimental maps at 3.3–4.8 Å resolution, and produces a nearly complete model...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nat Methods
Hauptverfasser: Wang, Ray Yu-Ruei, Kudryashev, Mikhail, Li, Xueming, Egelman, Edward H., Basler, Marek, Cheng, Yifan, Baker, David, DiMaio, Frank
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4435692/
https://ncbi.nlm.nih.gov/pubmed/25707029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3287
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