Acrosome Reaction and Ca(2+) Imaging in Single Human Spermatozoa: New Regulatory Roles of [Ca(2+)]i

The spermatozoa acrosome reaction (AR) is essential for mammalian fertilization. Few methods allow visualization of AR in real time together with Ca(2+) imaging. Here, we show that FM4-64, a fluorescent dye used to follow exocytosis, reliably reports AR progression induced by ionomycin and progester...

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Библиографические подробности
Опубликовано в: :Biol Reprod
Главные авторы: Sánchez-Cárdenas, Claudia, Servín-Vences, Martha Rocio, José, Omar, Treviño, Claudia Lydia, Hernández-Cruz, Arturo, Darszon, Alberto
Формат: Artigo
Язык:Inglês
Опубликовано: Society for the Study of Reproduction, Inc. 2014
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Articles
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4435061/
https://ncbi.nlm.nih.gov/pubmed/25100708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1095/biolreprod.114.119768
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