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Heterologous expression and characterization of laccase 2 from Coprinopsis cinerea capable of decolourizing different recalcitrant dyes

The gene (CcLcc2) encoding laccase from the basidiomycete Coprinopsis cinerea Okayama-7 #130 was synthesized by polymerase chain reaction-based two-step DNA synthesis, and heterologously expressed in Pichia pastoris. The recombinant protein was purified by ammonium sulphate precipitation and nickel...

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Detalhes bibliográficos
Publicado no:Biotechnol Biotechnol Equip
Main Authors: Tian, Yong-Sheng, Xu, Hu, Peng, Ri-He, Yao, Quan-Hong, Wang, Rong-Tan
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4433907/
https://ncbi.nlm.nih.gov/pubmed/26019510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/13102818.2014.913402
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