Monitoring protein phosphorylation by acrylamide pendant Phos-Tag™ in various plants

The aim of the present study is to rationalize acrylamide pendant Phos-Tag™ in-gel discrimination of phosphorylated and non-phosphorylated plant protein species with standard immunoblot analysis, and optimize sample preparation, efficient electrophoretic separation and transfer. We tested variants o...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Bekešová, Slávka, Komis, George, Křenek, Pavel, Vyplelová, Petra, Ovečka, Miroslav, Luptovčiak, Ivan, Illés, Peter, Kuchařová, Anna, Šamaj, Jozef
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2015
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4429547/
https://ncbi.nlm.nih.gov/pubmed/26029234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2015.00336
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