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Transfection of RNA from Organ Samples of Infected Animals Represents a Highly Sensitive Method for Virus Detection and Recovery of Classical Swine Fever Virus

Translation and replication of positive stranded RNA viruses are directly initiated in the cellular cytoplasm after uncoating of the viral genome. Accordingly, infectious virus can be generated by transfection of RNA genomes into susceptible cells. In the present study, efficiency of conventional vi...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Meyer, Denise, Schmeiser, Stefanie, Postel, Alexander, Becher, Paul
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4427382/
https://ncbi.nlm.nih.gov/pubmed/25961582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0126806
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