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Recombinant production, crystallization and crystal structure determination of dihydroorotate dehydrogenase from Leishmania (Viannia) braziliensis

The enzyme dihydroorotate dehydrogenase (DHODH) is a flavoenzyme that catalyses the oxidation of dihydroorotate to orotate in the de novo pyrimidine-biosynthesis pathway. In this study, a reproducible protocol for the heterologous expression of active dihydroorotate dehydrogenase from Leishmania (Vi...

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Vydáno v:Acta Crystallogr F Struct Biol Commun
Hlavní autoři: Reis, Renata Almeida Garcia, Lorenzato, Eder, Silva, Valeria Cristina, Nonato, Maria Cristina
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4427163/
https://ncbi.nlm.nih.gov/pubmed/25945707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15000886
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