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Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation

Colony formation in soft agar is the gold-standard assay for cellular transformation in vitro, but it is unsuited for high-throughput screening. Here, we describe an assay for cellular transformation that involves growth in low attachment (GILA) conditions and is strongly correlated with the soft-ag...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Rotem, Asaf, Janzer, Andreas, Izar, Benjamin, Ji, Zhe, Doench, John G., Garraway, Levi A., Struhl, Kevin
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4426412/
https://ncbi.nlm.nih.gov/pubmed/25902495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1505979112
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