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Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs

Recombineering is an in vivo genetic engineering technique involving homologous recombination mediated by phage recombination proteins. The use of recombineering methodology is not limited by size and sequence constraints and therefore has enabled the streamlined construction of bacterial strains an...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Reddy, Thimma R., Kelsall, Emma J., Fevat, Léna M. S., Munson, Sarah E., Cowley, Shaun M.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4425527/
https://ncbi.nlm.nih.gov/pubmed/25954970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0125533
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