Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs

Recombineering is an in vivo genetic engineering technique involving homologous recombination mediated by phage recombination proteins. The use of recombineering methodology is not limited by size and sequence constraints and therefore has enabled the streamlined construction of bacterial strains an...

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Dades bibliogràfiques
Publicat a:PLoS One
Autors principals: Reddy, Thimma R., Kelsall, Emma J., Fevat, Léna M. S., Munson, Sarah E., Cowley, Shaun M.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2015
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4425527/
https://ncbi.nlm.nih.gov/pubmed/25954970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0125533
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