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Integrating chemical mutagenesis and whole genome sequencing as a platform for forward and reverse genetic analysis of Chlamydia

Gene inactivation by transposon insertion or allelic exchange is a powerful approach to probe gene function. Unfortunately, many microbes, including Chlamydia, are not amenable to routine molecular genetic manipulations. Here we describe an arrayed library of chemically-induced mutants of the geneti...

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Bibliografski detalji
Izdano u:Cell Host Microbe
Glavni autori: Kokes, Marcela, Dunn, Joe Dan, Granek, Joshua A., Nguyen, Bidong D., Barker, Jeffrey R., Valdivia, Raphael H., Bastidas, Robert J.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4418230/
https://ncbi.nlm.nih.gov/pubmed/25920978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chom.2015.03.014
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