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Integrating chemical mutagenesis and whole genome sequencing as a platform for forward and reverse genetic analysis of Chlamydia
Gene inactivation by transposon insertion or allelic exchange is a powerful approach to probe gene function. Unfortunately, many microbes, including Chlamydia, are not amenable to routine molecular genetic manipulations. Here we describe an arrayed library of chemically-induced mutants of the geneti...
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| Publicado en: | Cell Host Microbe |
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| Autores principales: | , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4418230/ https://ncbi.nlm.nih.gov/pubmed/25920978 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chom.2015.03.014 |
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