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RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes
DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene. We find that the enzyme displays a rel...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4417176/ https://ncbi.nlm.nih.gov/pubmed/25824942 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv269 |
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