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Production of calves by transfer of nuclei from cultured inner cell mass cells.

We report here the isolation and in vitro culture of bovine inner cell mass (ICM) cells and the use of ICM cells in nuclear transfer to produce totipotent blastocysts that resulted in calves born. Of 15 cell lines represented in this study, 13 were derived from immunosurgically isolated ICM of 3 in...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Sims, M, First, N L
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1994
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44154/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8016127/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.13.6143
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