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Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull

Large macromolecular complexes such as the spliceosomal small nuclear ribonucleoproteins (snRNPs) play a variety of roles within the cell. Despite their biological importance, biochemical studies of snRNPs and other machines are often thwarted by practical difficulties in the isolation of sufficient...

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Pubblicato in:RNA
Autori principali: Rodgers, Margaret L., Paulson, Joshua, Hoskins, Aaron A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4408783/
https://ncbi.nlm.nih.gov/pubmed/25805862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.047845.114
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