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Simple Method for Markerless Gene Deletion in Multidrug-Resistant Acinetobacter baumannii
The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating gene deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of the PCR products in DNA cloning and the con...
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| Publicado en: | Appl Environ Microbiol |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4407223/ https://ncbi.nlm.nih.gov/pubmed/25746991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03975-14 |
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