Novel Hairpin-Shaped Primer Assay To Study the Association of the −44 Single-Nucleotide Polymorphism of the DEFB1 Gene with Early-Onset Periodontal Disease

A powerful, cost-effective new method for studying single-nucleotide polymorphisms (SNPs) is described. This method is based on the use of hairpin-shaped primers (HP), which give a sensitive and specific PCR amplification of each specific allele, without the use of costly fluorophore-labeled probes...

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Detalhes bibliográficos
Main Authors: Boniotto, Michele, Hazbón, Manzour Hernando, Jordan, William James, Lennon, Greig Patrick, Eskdale, Joyce, Alland, David, Gallagher, Grant
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Immune-Mediated Responses and Disorders
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC440632/
https://ncbi.nlm.nih.gov/pubmed/15242954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.11.4.766-769.2004
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