Effective amplification of long targets from cloned inserts and human genomic DNA.
We have used the polymerase chain reaction (PCR) to amplify up to 22 kb of the beta-globin gene cluster from human genomic DNA and up to 42 kb from phaga lambda DNA. We have also amplified 91 human genomic inserts of 9-23 kb directly from recombinant lambda plaques. To do this, we increased pH, adde...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44063/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202550/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.12.5695 |
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