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Isolation and Characterization of Thiamin Pyrophosphotransferase from Glycine max Seedlings

Thiamin:ATP pyrophosphotransferase (EC2.7.6.2) activity from soybean (Merr.) seedlings grown for 48 hours was determined by measuring the rate of [2-(14)C]thiamin incorporation into thiamin pyrophosphate. With partially purified (11-fold) enzyme, optimal activity occurred between pH 7.1 and 7.3, dep...

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Bibliografiske detaljer
Main Authors: Molin, William T., Fites, Roger C.
Format: Artigo
Sprog:Inglês
Udgivet: 1980
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC440588/
https://ncbi.nlm.nih.gov/pubmed/16661427
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