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Transcription in Isolated Wheat Nuclei: II. CHARACTERIZATION OF RNA SYNTHESIZED IN VITRO

Nuclei free of RNase activity were isolated from 3-hour-imbibed wheat (var. Yamhill) embryos by centrifugation through a discontinuous gradient of Percoll. The maximum rate of RNA synthesis observed in these nuclei was approximately 5 picomoles [(3)H]UTP per milligram DNA per minute. Two monovalent...

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Detalhes bibliográficos
Main Authors: Luthe, Dawn Sywassink, Quatrano, Ralph S.
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC440316/
https://ncbi.nlm.nih.gov/pubmed/16661179
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