Identification of New Genes Contributing to the Extreme Radioresistance of Deinococcus radiodurans Using a Tn5-Based Transposon Mutant Library

Here, we have developed an extremely efficient in vivo Tn5-based mutagenesis procedure to construct a Deinococcus radiodurans insertion mutant library subsequently screened for sensitivity to genotoxic agents such as γ and UV radiations or mitomycin C. The genes inactivated in radiosensitive mutants...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Dulermo, Rémi, Onodera, Takefumi, Coste, Geneviève, Passot, Fanny, Dutertre, Murielle, Porteron, Martine, Confalonieri, Fabrice, Sommer, Suzanne, Pasternak, Cécile
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2015
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4401554/
https://ncbi.nlm.nih.gov/pubmed/25884619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0124358
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