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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
It was recently demonstrated that recombinase polymerase amplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sample concentration using a standard curve. In this manuscript, a detailed protocol for developing and implementing a real-time quan...
Enregistré dans:
| Publié dans: | J Vis Exp |
|---|---|
| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4401391/ https://ncbi.nlm.nih.gov/pubmed/25867513 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52620 |
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