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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

It was recently demonstrated that recombinase polymerase amplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sample concentration using a standard curve. In this manuscript, a detailed protocol for developing and implementing a real-time quan...

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書目詳細資料
發表在:J Vis Exp
Main Authors: Crannell, Zachary A., Rohrman, Brittany, Richards-Kortum, Rebecca
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4401391/
https://ncbi.nlm.nih.gov/pubmed/25867513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52620
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