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Single and Multiphoton Fluorescence Recovery after Photobleaching

Fluorescence recovery after photobleaching (FRAP) is a microscopy technique for measuring the kinetics of fluorescently labeled molecules, and can be applied both in vitro and in vivo for two-and three-dimensional systems. This chapter discusses the three basic FRAP methods: traditional FRAP, multi-...

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Bibliographic Details
Published in:Cold Spring Harb Protoc
Main Authors: Sullivan, Kelley D., Majewska, Ania K., Brown, Edward B.
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4399237/
https://ncbi.nlm.nih.gov/pubmed/25561627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.top083519
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