Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs) are circularized DNA elements formed during recombination process that creates T- and B-cell receptors. Because TRECs and KRECs are unable to replicate, they are diluted after each cell division, and there...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Sottini, Alessandra, Serana, Federico, Bertoli, Diego, Chiarini, Marco, Valotti, Monica, Vaglio Tessitore, Marion, Imberti, Luisa
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396956/
https://ncbi.nlm.nih.gov/pubmed/25549107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52184
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