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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Centrosomes are small but important organelles that serve as the poles of mitotic spindle to maintain genomic integrity or assemble primary cilia to facilitate sensory functions in cells. The level of a protein may be regulated differently at centrosomes than at other .cellular locations, and the va...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4396923/ https://ncbi.nlm.nih.gov/pubmed/25548932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52030 |
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