Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin

We established two Madin-Darby canine kidney (MDCK) cell lines stably expressing human airway transmembrane protease: transmembrane protease, serine 2 (TMPRSS2) and mosaic serine protease large form (MSPL) which support multicycle growth of two H5 highly pathogenic avian influenza viruses (HPAIV) re...

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Veröffentlicht in:Biotechnol Res Int
Hauptverfasser: Wen, Zhiyuan, Wu, Chao, Chen, Weiye, Zeng, Xianying, Shi, Jianzhong, Ge, Jinying, Chen, Hualan, Bu, Zhigao
Format: Artigo
Sprache:Inglês
Veröffentlicht: Hindawi Publishing Corporation 2015
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396729/
https://ncbi.nlm.nih.gov/pubmed/25918647
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2015/402628
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