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Protein Helical Structure Determination Using CD Spectroscopy for Solutions with Strong Background Absorbance from 190-230 nm

Conventional empirical methods for the quantification of the helical content of proteins in solution using circular dichroism (CD) primarily rely on spectral data acquired between wavelengths of 190 to 230 nm. The presence of chemical species in a protein solution with strong absorbance within this...

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Detalhes bibliográficos
Publicado no:Biochim Biophys Acta
Main Authors: Wei, Yang, Thyparambil, Aby A., Latour, Robert A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4395514/
https://ncbi.nlm.nih.gov/pubmed/25308773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2014.10.001
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