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Assessment of SYBR Green I Dye-Based Fluorescence Assay for Screening Antimalarial Activity of Cationic Peptides and DNA Intercalating Agents

The SYBR green I (SG) dye-based fluorescence assay for screening antimalarial compounds is based on direct quantitation of parasite DNA. We show that DNA-interacting cationic cell-penetrating peptides (CPPs) and intercalating agents compete with SG dye to bind to DNA. Therefore, readouts of this ass...

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Publicat a:Antimicrob Agents Chemother
Autors principals: Bhatia, Rakesh, Gautam, Ankur, Gautam, Shailendra K., Mehta, Divya, Kumar, Vinod, Raghava, Gajendra P. S., Varshney, Grish C.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4394797/
https://ncbi.nlm.nih.gov/pubmed/25691642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.03266-14
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