An improved protocol to study the plant cell wall proteome

Cell wall proteins were extracted from alfalfa stems according to a three-steps extraction procedure using sequentially CaCl(2), EGTA, and LiCl-complemented buffers. The efficiency of this protocol for extracting cell wall proteins was compared with the two previously published methods optimized for...

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Bibliografiske detaljer
Udgivet i:Front Plant Sci
Main Authors: Printz, Bruno, Dos Santos Morais, Raphaël, Wienkoop, Stefanie, Sergeant, Kjell, Lutts, Stanley, Hausman, Jean-Francois, Renaut, Jenny
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2015
Fag:
Plant Science
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4392696/
https://ncbi.nlm.nih.gov/pubmed/25914713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2015.00237
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