Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification

A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay e...

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Veröffentlicht in:J Clin Microbiol
Hauptverfasser: Teoh, Boon-Teong, Sam, Sing-Sin, Tan, Kim-Kee, Danlami, Mohammed Bashar, Shu, Meng-Hooi, Johari, Jefree, Hooi, Poh-Sim, Brooks, David, Piepenburg, Olaf, Nentwich, Oliver, Wilder-Smith, Annelies, Franco, Leticia, Tenorio, Antonio, AbuBakar, Sazaly
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2015
Schlagworte:
Virology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4390637/
https://ncbi.nlm.nih.gov/pubmed/25568438
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02648-14
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