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Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR

We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to i...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:J Clin Microbiol
Egile Nagusiak: Edouard, Sophie, Prudent, Elsa, Gautret, Philippe, Memish, Ziad A., Raoult, Didier
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology 2015
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4390636/
https://ncbi.nlm.nih.gov/pubmed/25552360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.03609-14
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