Dual-view plane illumination microscopy for rapid and spatially isotropic imaging

We describe the construction and use of a compact dual-view inverted selective plane illumination microscope (diSPIM) for time-lapse volumetric (4D) imaging of living samples at subcellular resolution. Our protocol enables a biologist with some prior microscopy experience to assemble a diSPIM from c...

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Udgivet i:Nat Protoc
Main Authors: Kumar, Abhishek, Wu, Yicong, Christensen, Ryan, Chandris, Panagiotis, Gandler, William, McCreedy, Evan, Bokinsky, Alexandra, Colón-Ramos, Daniel A, Bao, Zhirong, McAuliffe, Matthew, Rondeau, Gary, Shroff, Hari
Format: Artigo
Sprog:Inglês
Udgivet: 2014
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4386612/
https://ncbi.nlm.nih.gov/pubmed/25299154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.172
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