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Ultrastructurally-smooth thick partitioning and volume stitching for larger-scale connectomics
FIB-SEM has become an essential tool for studying neural tissue at resolutions below 10×10×10 nm, producing datasets superior for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 µm thick) optimally...
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| Publié dans: | Nat Methods |
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| Auteurs principaux: | , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4382383/ https://ncbi.nlm.nih.gov/pubmed/25686390 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3292 |
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