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Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in Tetrahymena thermophila.

Replacement of lysine-350 by methionine in the beta-tubulin gene of Chlamydomonas confers resistance to microtubule-depolymerizing drugs and increased sensitivity to the microtubule-stabilizing drug taxol. This mutation was created in cloned BTU1, one of two coexpressed beta-tublin genes of Tetrahym...

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Hlavní autoři: Gaertig, J, Thatcher, T H, Gu, L, Gorovsky, M A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1994
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC43823/
https://ncbi.nlm.nih.gov/pubmed/7910408
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