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Recovery of Murine Norovirus and Feline Calicivirus from Plasmids Encoding EMCV IRES in Stable Cell Lines Expressing T7 Polymerase
Reverse genetics systems constitute one of the most important and powerful tools to study the molecular biology of viruses. We developed a new strategy for the recovery of murine norovirus from a single plasmid in which a bacteriophage T7 RNA polymerase (T7pol) promoter for transcription and an EMCV...
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| 發表在: | J Virol Methods |
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| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2015
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4380611/ https://ncbi.nlm.nih.gov/pubmed/25698463 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2015.02.003 |
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