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Recovery of Murine Norovirus and Feline Calicivirus from Plasmids Encoding EMCV IRES in Stable Cell Lines Expressing T7 Polymerase

Reverse genetics systems constitute one of the most important and powerful tools to study the molecular biology of viruses. We developed a new strategy for the recovery of murine norovirus from a single plasmid in which a bacteriophage T7 RNA polymerase (T7pol) promoter for transcription and an EMCV...

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Pubblicato in:J Virol Methods
Autori principali: Sandoval-Jaime, Carlos, Green, Kim Y., Sosnovtsev, Stanislav V.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4380611/
https://ncbi.nlm.nih.gov/pubmed/25698463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2015.02.003
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