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Heme-thiolate ferryl of aromatic peroxygenase is basic and reactive

A kinetic and spectroscopic characterization of the ferryl intermediate (APO-II) from APO, the heme-thiolate peroxygenase from Agrocybe aegerita, is described. APO-II was generated by reaction of the ferric enzyme with metachloroperoxybenzoic acid in the presence of nitroxyl radicals and detected wi...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Wang, Xiaoshi, Ullrich, René, Hofrichter, Martin, Groves, John T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4378415/
https://ncbi.nlm.nih.gov/pubmed/25759437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1503340112
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