Room-Temperature Distance Measurements of Immobilized Spin-Labeled Protein by DEER/PELDOR

Nitroxide spin labels are used for double electron-electron resonance (DEER) measurements of distances between sites in biomolecules. Rotation of gem-dimethyls in commonly used nitroxides causes spin echo dephasing times (T(m)) to be too short to perform DEER measurements at temperatures between ∼80...

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Publicat a:Biophys J
Autors principals: Meyer, Virginia, Swanson, Michael A., Clouston, Laura J., Boratyński, Przemysław J., Stein, Richard A., Mchaourab, Hassane S., Rajca, Andrzej, Eaton, Sandra S., Eaton, Gareth R.
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2015
Matèries:
Proteins and Nucleic Acids
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4375670/
https://ncbi.nlm.nih.gov/pubmed/25762332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.01.015
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