Multiple Mass Isotopomer Tracing of Acetyl-CoA Metabolism in Langendorff-perfused Rat Hearts: CHANNELING OF ACETYL-CoA FROM PYRUVATE DEHYDROGENASE TO CARNITINE ACETYLTRANSFERASE

We developed an isotopic technique to assess mitochondrial acetyl-CoA turnover (≈citric acid flux) in perfused rat hearts. Hearts are perfused with buffer containing tracer [(13)C(2),(2)H(3)]acetate, which forms M5 + M4 + M3 acetyl-CoA. The buffer may also contain one or two labeled substrates, whic...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Li, Qingling, Deng, Shuang, Ibarra, Rafael A., Anderson, Vernon E., Brunengraber, Henri, Zhang, Guo-Fang
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2015
Assuntos:
Metabolism
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4375469/
https://ncbi.nlm.nih.gov/pubmed/25645937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.631549
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