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Forging Isopeptide Bonds Using Thiol–Ene Chemistry: Site-Specific Coupling of Ubiquitin Molecules for Studying the Activity of Isopeptidases
Chemical methods for modifying proteins can enable studies aimed at uncovering biochemical function. Herein, we describe the use of thiol–ene coupling (TEC) chemistry to report on the function of branched (also referred to as forked) ubiquitin trimers. We show how site-specific isopeptide (Nε-Gly-l-...
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| Publicat a: | J Am Chem Soc |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4373626/ https://ncbi.nlm.nih.gov/pubmed/22497214 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja300500a |
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