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Robust microarray production of freshly expressed proteins in a human milieu

PURPOSE: In vitro transcription/translation (IVTT) systems are widely used in proteomics. For clinical applications, mammalian systems are preferred for protein folding and activity; however, the level of protein obtained is low. A new system extracted from human cells (1-Step Human Coupled IVT) has...

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Detalhes bibliográficos
Publicado no:Proteomics Clin Appl
Main Authors: Festa, Fernanda, Rollins, Sean M., Vattem, Krishna, Hathaway, Margarita, Lorenz, Phillip, Mendoza, Eliseo Alejandro, Yu, Xiaobo, Qiu, Ji, Kilmer, Greg, Jensen, Penny, Webb, Brian, Ryan, Ed T., LaBaer, Joshua
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4369765/
https://ncbi.nlm.nih.gov/pubmed/23027544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prca.201200063
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