Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization.

We describe a general strategy for cloning mammalian genes whose downregulation results in a selectable phenotype. This strategy is based on expression selection of genetic suppressor elements (GSEs), cDNA fragments encoding either specific peptides that act as dominant inhibitors of protein functio...

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Detalhes bibliográficos
Main Authors: Gudkov, A V, Kazarov, A R, Thimmapaya, R, Axenovich, S A, Mazo, I A, Roninson, I B
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC43658/
https://ncbi.nlm.nih.gov/pubmed/8170981
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