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Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis

Zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system are potentially powerful tools for producing tailor-made knockout animals. However, their mutagenic activit...

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Detalhes bibliográficos
Publicado no:Biol Open
Main Authors: Nakajima, Keisuke, Yaoita, Yoshio
Formato: Artigo
Idioma:Inglês
Publicado em: The Company of Biologists 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4365486/
https://ncbi.nlm.nih.gov/pubmed/25596277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.201410009
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