Sensitive Targeted Quantification of ERK Phosphorylation Dynamics and Stoichiometry in Human Cells without Affinity Enrichment

[Image: see text] Targeted mass spectrometry is a promising technology for site-specific quantification of posttranslational modifications. However, a major constraint is the limited sensitivity for quantifying low-abundance PTMs, requiring the use of affinity reagents for enrichment. Herein, we dem...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Shi, Tujin, Gao, Yuqian, Gaffrey, Matthew J., Nicora, Carrie D., Fillmore, Thomas L., Chrisler, William B., Gritsenko, Marina A., Wu, Chaochao, He, Jintang, Bloodsworth, Kent J., Zhao, Rui, Camp, David G., Liu, Tao, Rodland, Karin D., Smith, Richard D., Wiley, H. Steven, Qian, Wei-Jun
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4365449/
https://ncbi.nlm.nih.gov/pubmed/25517423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac503797x
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