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Analysis of a temperature-sensitive mutation in Uba1: Effects of the click reaction on subsequent immunolabeling of proteins involved in DNA replication

In our previous study, a Met-to-Ile substitution at amino acid 256 in the catalytic domain of Uba1 was determined in temperature-sensitive CHO-K1 mutant tsTM3 cells, which exhibited chromosomal instability and cell-cycle arrest in the S to G(2) phases with decreased DNA synthesis at the nonpermissiv...

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Bibliografiset tiedot
Julkaisussa:FEBS Open Bio
Päätekijät: Sugaya, Kimihiko, Ishihara, Yoshie, Inoue, Sonoe
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2015
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4359972/
https://ncbi.nlm.nih.gov/pubmed/25834782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2015.02.004
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