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Production and Discovery of Novel Recombinant Adeno-Associated Viral Vectors
In this unit, we describe the detailed protocol of a three-plasmid transfection method for rAAV production, its advantages, limitations, and troubleshooting techniques. We further discuss the rAAV purification process using CsCl gradients and the subsequent quality control steps by SDS-PAGE and rtPC...
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| I publikationen: | Curr Protoc Microbiol |
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| Huvudupphovsmän: | , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
2012
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4358842/ https://ncbi.nlm.nih.gov/pubmed/22875565 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780471729259.mc14d01s26 |
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