Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system

BACKGROUND: Two overlapping genes lacL and lacM (lacLM) encoding for heterodimeric β-galactosidase from Lactobacillus reuteri were previously cloned and over-expressed in the food-grade host strain Lactobacillus plantarum WCFS1, using the inducible lactobacillal pSIP expression system. In this study...

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Bibliografiske detaljer
Udgivet i:Microb Cell Fact
Main Authors: Nguyen, Tien-Thanh, Nguyen, Hoang-Minh, Geiger, Barbara, Mathiesen, Geir, Eijsink, Vincent GH, Peterbauer, Clemens K, Haltrich, Dietmar, Nguyen, Thu-Ha
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2015
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4358714/
https://ncbi.nlm.nih.gov/pubmed/25880197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0214-8
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