Mycobacterial RNA isolation optimized for non-coding RNA: high fidelity isolation of 5S rRNA from Mycobacterium bovis BCG reveals novel post-transcriptional processing and a complete spectrum of modified ribonucleosides

A major challenge in the study of mycobacterial RNA biology is the lack of a comprehensive RNA isolation method that overcomes the unusual cell wall to faithfully yield the full spectrum of non-coding RNA (ncRNA) species. Here, we describe a simple and robust procedure optimized for the isolation of...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Hia, Fabian, Chionh, Yok Hian, Pang, Yan Ling Joy, DeMott, Michael S., McBee, Megan E., Dedon, Peter C.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2015
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4357692/
https://ncbi.nlm.nih.gov/pubmed/25539917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1317
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