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Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Amphibian eggs have been widely used to study embryonic development. Early embryonic development is driven by maternally stored factors accumulated during oogenesis. In order to study roles of such maternal factors in early embryonic development, it is desirable to manipulate their functions from th...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Miyamoto, Kei, Simpson, David, Gurdon, John B.
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354630/
https://ncbi.nlm.nih.gov/pubmed/25742326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52496
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