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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
The ability to study biomolecules in vivo is crucial for understanding their function in a biological context. One powerful approach involves fusing molecules of interest to fluorescent proteins such as GFP to study their expression, localization and function. However, GFP and its derivatives are si...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4354625/ https://ncbi.nlm.nih.gov/pubmed/25741968 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52208 |
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