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Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Protein stability influences many aspects of biology, and measuring the clearance kinetics of proteins can provide important insights into biological systems. In FDAP experiments, the clearance of proteins within living organisms can be measured. A protein of interest is tagged with a photoconvertib...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Rogers, Katherine W., Bläßle, Alexander, Schier, Alexander F., Müller, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354555/
https://ncbi.nlm.nih.gov/pubmed/25650549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52266
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