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Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Discs large (Dlg) is a conserved member of the membrane-associated guanylate kinase family, and serves as a major scaffolding protein at the larval neuromuscular junction (NMJ) in Drosophila. Previous studies have shown that the postsynaptic distribution of Dlg at the larval NMJ overlaps with that o...

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Foilsithe in:J Vis Exp
Main Authors: Wang, Simon, Yoo, SooHyun, Kim, Hae-yoon, Wang, Mannan, Zheng, Clare, Parkhouse, Wade, Krieger, Charles, Harden, Nicholas
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354543/
https://ncbi.nlm.nih.gov/pubmed/25650626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52139
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